PEG 6000模拟干旱胁迫下四个园林小菊品种抗旱性评价

PEG 6000模拟干旱胁迫下四个园林小菊品种抗旱性评价

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]卷:期数:2024年23页码:48-57栏目:出版日期:2024-12-15

Title:
Evaluation of Drought Resistance of Four Garden Chrysanthemum Varieties Under Simulated Drought Stress With PEG 6000

文章编号:
S 682.31

分类号:
1001-0009(2024)23-0048-10

DOI:
10.11937/bfyy.20242232

文献标志码:
A

摘要:
以4个园林小菊品种盆栽为试材，采用PEG 6000模拟干旱胁迫法，研究了不同浓度梯度（10%、15%、20%和25%）对园林小菊生长指标、光合指标和生理指标的影响,以期为建立园林小菊抗旱评价体系提供参考依据。结果表明：干旱胁迫对园林小菊株高和生物量均有明显抑制作用。PEG 6000胁迫浓度升高至25%时，4个园林小菊品种光合指标净化合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）均下降至最低值；胞间CO2浓度（Ci）总体呈现先升后降的变化趋势；相对电导率、丙二醛（MDA）、脯氨酸（Pro）、可溶性糖含量和过氧化物酶（POD）活性均上升至最大值，表明园林小菊细胞膜的受损程度在不断加剧。‘红珍珠’‘名流千秋’和‘北美红’的可溶性蛋白质含量和超氧化物歧化酶（SOD）活性呈现先升高后下降的趋势；‘红珍珠’和‘名流千秋’的过氧化氢酶（CAT）活性呈现先升后降的变化趋势。4个园林小菊品种的抗旱性依次为‘黄金时代’>‘红珍珠’>‘北美红’>‘名流千秋’。

Abstract:
Four varieties of garden Chrysanthemum were used as the test materials，and PEG 6000 was used to simulate drought stress.The effects of different concentration gradients (10%，15%，20%，and 25%) on the growth，photosynthetic，and physiological indicators of Chrysanthemum were studied,in order to provide reference for establishing a drought resistance evaluation system for garden Chrysanthemum.The results showed that drought stress significantly suppressed plant height and biomass.At a PEG 6000 concentration of 25%，the Pn，Tr and Gs of all four cultivars reached their lowest values.Ci generally exhibited an initial increase followed by a decrease.Relative electrical conductivity，MDA，Pro，soluble sugar contents，and POD activity all increased significantly，indicating an escalating level of cell membrane damage in Chrysanthemum plants.The levels of soluble protein and SOD in ‘Hong Zhenzhu’‘Mingliu Qianqiu’ and ‘Beimeihong’ initially rose before declining.CAT activity in ‘Hong Zhenzhu’ and ‘Mingliu Qianqiu’ also displayed an initial increase followed by a decrease.The drought resistance ranking among the four garden Chrysanthemum varieties was as follows,‘Huangjin Shidai’>‘Hong Zhenzhu’>‘Beimeihong’>‘Mingliu Qianqiu’.

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